Salmonella is one of the most important zoonotic diseases affecting humans. The most important factor for salmonella as a very widespread pathogen is its ability to adapt to any host. Food contaminated with salmonella is a major source of human infection. The control program is also based on serological monitoring and salmonella isolation, not only to reduce the prevalence of infection, but as a valuable tool to change routine procedures to reduce food contamination.
Salmonella enteritis (SE), an important pathogen in poultry, has been isolated from broiler, breeding and commercial laying hens. Because of the intermittent excretion of bacteria, it is difficult to isolate and identify positive birds. The presence of antibodies does not always mean infection, but it is a sign of previous infection.
Enzyme-linked immunosorbent assay (ELISA) is effective in detecting salmonella antibodies in poultry, especially in large populations of salmonella enteritis.
Primary screening of antibodies against salmonella enteritis. Since SE ELISA is based on the detection of gm flagella protein, other serotype salmonella bacteria may also have positive results due to the presence of gm flagella protein antigen-determining clusters. Therefore, positive ELISA monitoring results must be confirmed by standardized bacteriological detection methods.
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