Salmonella is a pathogenic bacterium, so in our country, its limit in food is 0, once detected, it is an unqualified product, not allowed to enter the market.
1. Traditional national standard method of bacterial culture: after the inoculation, the samples were cultured through selective medium, and finally the samples were determined by visual observation to determine whether there was salmonella, and the samples could be further serologically classified by antiserum.
Advantages: low cost and accurate results
Disadvantages: high requirements on operators' professional skills and experience, high testing time cost, usually more than 4 days to get results.
2. ELISA kit method: after the immunological antigen antibody reaction, the results were interpreted by the naked eye or the instrument through enzyme catalyzed color rendering or luminescence.
Advantages: moderate cost, accurate results, strong specificity, high sensitivity, easy operation
Weakness: have certain professional skill requirement to operator
3. PCR method: PCR uses molecular biology technology to make DNA replicate rapidly in vitro in accordance with the principle of base complementary pairing through temperature and enzyme catalysis, and then dyes the DNA and interprets the results through the instrument.
Advantages: high sensitivity, high specificity, simple and accurate result analysis, low detection time cost and easy automation
Disadvantages: high cost, complex experimental operation and easy pollution
Rt-pcr method: realtime-pcr is to add fluorescent groups/probes into the traditional PCR reaction system, and use the accumulation of fluorescence signals to detect the entire PCR process in RealTime. Finally, qualitative and quantitative analysis of gene sequences can be realized through standard curve analysis. It retains the characteristics of high sensitivity and specificity of PCR, and at the same time, it can carry out complete quantitative detection and shorten the detection time.
salmonella rapid test kit is of high quality. Learn more about the salmonella rapid test kit
from chemtron biotech.